A colorimetric method for DNA hybridization
نویسندگان
چکیده
منابع مشابه
A colorimetric method for DNA hybridization.
A general method has been developed which allows crosslinks to be produced between proteins and single-stranded DNA. Such single-stranded DNA protein complexes have been tested for blot hybridization using two colorimetrically detectable enzymes, namely peroxidase and alkaline phosphatase, as the protein moiety of the probe. After hybridization and incubation with a substrate solution sequences...
متن کاملIdentification of Salmonella enteritidis from experimentally infected hens using a colorimetric DNA hybridization method.
Identification of Salmonella enteritidis from cloacally challenged commercial laying hens was studied by comparing bacterial isolations using conventional methods with detection by the use of the GENE-TRAK colorimetric DNA probe assay. More positive test results were obtained using the latter on days 14, 28, and 42 postchallenge, but the difference between the two methods was not statistically ...
متن کاملEvaluation of a new fluorimetric DNA-DNA hybridization method.
Standardized procedures must be followed when characterizing, officially describing, and validly naming novel bacteria. For species descriptions, DNA-DNA hybridization still is needed for whole-genome comparisons between close relatives, but many established hybridization methods have drawbacks, such as requiring labeled or large amounts of DNA. We evaluated a new technique based on the spectro...
متن کاملDNA Nanotubes Coupled with Magnetic Nanoparticles as a Platform for Colorimetric Biosensors
This study describes the fabrication techniques for two forms of magnetic DNA nanotubes (MDNTs) and their applications as platforms for developing colorimetric assays. The first form of MDNTs was DNTs filled-up with magnetic nanoparticles (MNPs) and the second one was DNTs arayed with MNPs on their extrior surfaces. Then the both forms of MDNTs were employed as platforms for attaching a specifi...
متن کاملNonradioactive, colorimetric microplate hybridization assay for detecting amplified human immunodeficiency virus DNA.
A nonradioactive, colorimetric microplate hybridization procedure was used to assay human immunodeficiency virus (HIV) DNA, amplified by the polymerase chain reaction (PCR). Under the PCR conditions used, four proviral copies per 150,000 cells were detected by amplifying a series of DNA mixtures that contained various copy numbers of HIV. Assays of PCR-amplified DNA from peripheral blood mononu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1984
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/12.8.3435